Counting Isolated Nuclei on a CellDrop | Tech Team Tips скачать в хорошем качестве

Counting Isolated Nuclei on a CellDrop | Tech Team Tips

In this video, DeNovix Application Scientist Ben Capozzoli explains how to count single nuclei suspensions on the CellDrop Automated Cell Counter. Isolating nuclei is critical for a number of workflows, including ATAC-sequencing, RNA-sequencing, and a variety of others. To ensure successful results in downstream applications, it's important to have accurate counts of your nuclei and understand how much debris is in your sample. The CellDrop efficiently determines nuclei counts as well as an extraction efficiency score, regardless of the presence of debris. Watch to learn more about how this process works. Experience the CellDrop Free Trial Program https://www.denovix.com/free-trial/?u... Schedule a Virtual Demo https://www.denovix.com/virtual-demo/... Resources Tech Note 216: Counting Isolated Nuclei Using a CellDrop (detailed protocol, list of tissue types, etc.) https://www.denovix.com/tn-216-counti... Tech Note 186: CellDrop Best Practices (loading, adjusting focus & exposure, cleaning, etc.) https://www.denovix.com/tn-186-celldr... CellDrop Protocol Settings (list of pre-configured protocols for different cell types) https://www.denovix.com/products/cell... Contact us at info@denovix.com if you have any questions. Chapters: 00:00 Introduction 00:46 The CellDrop Automated Cell Counter 01:15 Preparing the Nuclei Suspension 02:05 AO/PI Viability Dye 02:40 CellDrop Count & Results 03:46 Cleaning 03:58 Outro