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Watch the Full Video at https://www.jove.com/v/2685/an-optic-.... An Optic Nerve Crush Injury Murine Model to Study Retinal Ganglion Cell Survival - a 2 minute Preview of the Experimental Protocol Zhongshu Tang, Shuihua Zhang, Chunsik Lee, Anil Kumar, Pachiappan Arjunan, Yang Li, Fan Zhang, Xuri Li NIH, National Eye Institute; The Second Hospital of Harbin Medical University, Ophthalmology Department; This protocol shows how to retrogradely label retinal ganglion cells, and how to subsequently make an optic nerve crush injury in order to analyze retinal ganglion cell survival and apoptosis. It is an experimental disease model for different types of optic neuropathy, including glaucoma. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments