Extracting Plasmid DNA by the Alkaline Lysis Method (BIOL310) скачать в хорошем качестве

Extracting Plasmid DNA by the Alkaline Lysis Method (BIOL310)

In this video, I take you step-by-step through the entire procedure for a typical "plasmid mini prep". Throughout the video, I point out some specific things you should be doing and should be observing. 00:32 - Preparation is important 01:19 - What's in your ice bucket 02:05 - The Phenol/Chloroform tube 02:55 - Balancing the tubes in a microfuge 03:55 - End of first centrifugation 04:55 - Removing the LB from the pelleted cells 06:45 - Vortexing your samples to resuspend the cell pellets (set the vortex to "Touch" and maximum speed) 08:15 - Addition of Solution B and observations 10:05 - Addition of Solution C and observations 12:40 - Recommended orientation of epi tubes when centrifuging samples 13:30 - Separation of supernatant from the pellet 16:35 - Addition of Phenol/Chloroform (work quickly) 19:20 - Separating the aqueous phase from the organic phase 19:34 - Why am I wearing gloves? 20:55 - Transferring out the aqueous phase 24:45 - DNA precipitation 28:20 - DNA pellets! 31:09 - Ethanol wash 33:05 - Drying your DNA pellets