AliveFRESH: Producing geraniol from C. reinhardtii скачать в хорошем качестве

AliveFRESH: Producing geraniol from C. reinhardtii

Climate change has been an ongoing global crisis and continues to worsen each year. In the last ten years, global temperatures have reached an all-time high. Additionally, burning fossil fuels and the emission of heat-trapping greenhouse gases have hastened climate change. These emissions include methane, nitrogen oxides, and carbon dioxide, which make up the greatest percentage of greenhouse gases. The most abundant greenhouse gas is carbon dioxide (CO₂). CO2 has been produced by many factors related directly to humans, including the products of industrial factories and gas-powered vehicles. To combat this issue, AliveFRESH proposes a biological system that absorbs carbon dioxide while simultaneously producing a pleasant scent. Using a natural air freshener, can avoid the negative impacts on human health, including the production of volatile organic compounds. The autotrophic single-celled organism, Chlamydomonas reinhardtii, would be engineered to produce geraniol, a monoterpene with a floral scent. C. reinhardtii was selected for this project because it is photosynthetic and uses a native methylerythritol phosphate (MEP) pathway. The light-independent reactions of photosynthesis will absorb carbon dioxide and produce G3P, a precursor to MEP. By utilizing the existing MEP pathway, AliveFRESH will produce isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP), which can convert into geranyl diphosphate (GPP) for subsequent monoterpene production. The final plasmid will include geraniol synthase (GES) and a red fluorescent protein (RFP) gene. With the addition of GES, GPP would convert into geraniol. RFP can be used to determine whether the transformation was successful by placing the culture of transformed algae under ultraviolet light. The AliveFRESH project creates a pleasant fragrance while also consuming carbon dioxide from the atmosphere. The information can be used to create a working plasmid through Addgene to be transformed in the future and is a potential solution to reduce the effects of climate change. Visit http://biotreks.org/e202208/ to learn more.