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Watch the Full Video at https://www.jove.com/v/55892/high-thr.... High-throughput Analysis of Locomotor Behavior in the Drosophila Island Assay - a 2 minute Preview of the Experimental Protocol Ilse Eidhof, Michaela Fenckova, Dei M. Elurbe, Bart van de Warrenburg, Anna Castells Nobau, Annette Schenck Radboud University Medical Center, Department of Human Genetics, Donders Institute for Brain, Cognition and Behaviour; Radboud University Medical Center, Centre for Molecular and Biomolecular Informatics, Radboud Institute for Molecular Life Sciences; Radboud University Medical Center, Department of Neurology, Donders Institute for Brain, Cognition and Behaviour; The island assay is a relatively new, cost-effective assay that can be used to evaluate the basic locomotor behavior of Drosophila melanogaster. This manuscript describes algorithms for automatic data processing and objective quantification of island assay data, making this assay a sensitive, high-throughput readout for large genetic or pharmacological screens. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments