DNA Cloning - how to overcome some common problems скачать в хорошем качестве

DNA Cloning - how to overcome some common problems

This video explores some common problems when cloning DNA and how to overcome them. 👉 Next video in the cell biology series -    • Understanding Nucleic Acid Hybridisation: ...   🌎 A free fact sheet covering the key points and definitions is available at: https://cell.drnickmorris.com/dna_clo... In this video, I break down the step-by-step process of cloning DNA into plasmids and address common challenges faced in the lab. Starting with the preparation of the DNA, I move through the use of restriction enzymes like EcoRI and HindIII and explain the importance of choosing the correct enzyme pairs to prevent self-ligation and ensure the correct orientation of the insert. I also cover the blue-white selection method to verify successful cloning and discuss using different vectors for larger DNA segments. Additional Resources This video was produced with help from the following resources: 📗 - The Biosciences Glossary - https://play.google.com/store/books/d... 📗 - Molecular Biology of the Cell (Alberts) - https://amzn.to/3zDyhQu (affiliate link) 📗 - Molecular Cell Biology (Lodish) - https://amzn.to/3zyIJZH (affiliate link) 📗 - Biochemistry (Stryer) - https://amzn.to/3XJ7gTR (affiliate link) 🌎 - A free fact sheet covering the key points and definitions is available at: https://cell.drnickmorris.com/dna_clo... 🫖 - If you would like to say thanks for the video, then please feel free to Buy me a coffee - https://www.buymeacoffee.com/drnickm Please don’t forget to subscribe so you know when I post more content. And don't forget to share with your classmates. If you have any questions, please post a comment. #dnacloning #cloning #cloningProblems #geneticengineering #molecularbiology #labskills #plasmid