Developing crosslinking mass spectrometry - Dr Francis O'Reilly (Technische Universität Berlin) скачать в хорошем качестве

Developing crosslinking mass spectrometry - Dr Francis O'Reilly (Technische Universität Berlin)

Developing crosslinking mass spectrometry towards structural proteomics inside cells Crosslinking mass spectrometry can identify residues that are nearby in space and has been used fairly routinely to study the topology of protein complexes in vitro. During my postdoc I have tried to develop this technology towards application to the entire proteome of cells. This can allow us to study the topology of protein interactions that are not stable ex vivo. I will discuss our progress in this direction and talk about our recent paper where we discovered a novel bacterial transcription-translation coupling complex. We combined in cell cross-linking mass spectrometry and cryo-electron tomography to identify and structurally characterise an indirect relationship between the RNA polymerase and the ribosome bridged through NusA, an anti-termination factor ubiquitous in bacteria.