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Watch the Full Video at https://www.jove.com/v/58744/stable-d.... Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules - a 2 minute Preview of the Experimental Protocol Xin Guo, Xue-Mei Wang, Shou-Jun Xiao Nanjing University, State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering; This article presents a detailed protocol for T4 ligation and denaturing PAGE purification of small circular DNA molecules, annealing and native PAGE analysis of circular tiles, assembling and AFM imaging of 1D and 2D DNA nanostructures, as well as agarose gel electrophoresis and centrifugation purification of finite DNA nanostructures. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments