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Watch the Full Video at https://www.jove.com/v/55028/establis.... Establishment of Proliferative Tetraploid Cells from Nontransformed Human Fibroblasts - a 2 minute Preview of the Experimental Protocol Susumu Ohshima, Atsushi Seyama Saitama Medical University, Division of Morphological Science, Biomedical Research Center; Saitama Medical University, Department of Pathology, International Medical Center; Although proliferative polyploid cells are necessary to analyze chromosomal instability of polyploid cells, creating such cells from nontransformed human cells is not easy. The present report describes relatively simple procedures to establish proliferative tetraploid cells free of a diploid population from normal human fibroblasts. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments