Industrial Fermentation: Productivity, Variability, and Scale Translation скачать в хорошем качестве

Industrial Fermentation: Productivity, Variability, and Scale Translation

In this episode we discuss transition of microbial fermentation from controlled laboratory settings to the complex realities of industrial manufacturing. It characterizes productivity not as a simple biological trait, but as an emergent property shaped by mechanical constraints like oxygen transfer limits, non-ideal mixing, and equipment drift. I would like to emphasize that performance often declines at scale because large vessels create spatial gradients and environmental oscillations that small-scale reactors do not replicate. Successful scale translation requires prioritizing operational resilience and robustness over the pursuit of maximum theoretical yields. Ultimately, the sources argue for using scale-down modeling and conservative design to align biological potential with the inevitable engineering variability of a factory environment. #Bioprocess #ScaleUp and #TechTransfer, #Industrial #Microbiology, #MetabolicEngineering and #SystemsBiology, #Bioprocessing, #MicrobialFermentation, #Bio-manufacturing, #Industrial #Biotechnology, #Fermentation Engineering, #ProcessDevelopment, #Microbiology, #Biochemistry #Biochemical Engineering, #Applied #MicrobialPhysiology, #Microbial #ProcessEngineering, #Upstream #BioprocessDevelopment, #Downstream Processing and #Purification, #CellCulture and #MicrobialSystems Engineering, #Bioreaction #Enzymes #Biocatalyst #scientific #Scientist #Research _____________________ Timestamp Timestamp Problem Addressed 01:54–02:26 The "Laboratory Lie" (The false assumption of homogeneity). 04:52–06:04 Stochastic failure of high-titer strains due to metabolic shunting. 07:42–08:26 OTR/OUR Mismatch causing localized cellular starvation. 10:13–11:32 The Scale Translation Paradox (Impossible parameter preservation). 12:22–12:54 Misdiagnosis of strain instability vs. environmental stress. 13:42–15:35 Cumulative Batch Drift (Facility "Memory"). 17:01–17:31 Pilot plant inefficiency (Using pilot runs only for verification).