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Watch the Full Video at https://www.jove.com/v/59220/a-human-.... A Human Blood-Brain Interface Model to Study Barrier Crossings by Pathogens or Medicines and Their Interactions with the Brain - a 2 minute Preview of the Experimental Protocol Anaelle da Costa, Christophe Prehaud, Florian Bakoa, Philippe Afonso, Pierre-Emmanuel Ceccaldi, Pierre Lafaye, Monique Lafon Institut Pasteur, CNRS UMR 3569, Unité de Neuroimmunologie Virale,; Institut Pasteur, Unité d’Epidémiologie et de Pathophysiologie des Virus Oncogènes, Université Sorbonne Paris Cité/Paris Diderot,; Institut Pasteur, PFIA, DTPS,; Here we present a protocol describing the setting of an in cellulo BBB (Blood brain barrier)-Minibrain polyester porous membrane culture insert system in order to evaluate the transport of biomolecules or infectious agents across a human BBB and their physiological impact on the neighboring brain cells. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments