Antimicrobial susceptibility testing for Staphylococcus ; Methods and Antibiotics Used in Testing скачать в хорошем качестве

Antimicrobial susceptibility testing for Staphylococcus ; Methods and Antibiotics Used in Testing

Antimicrobial susceptibility testing (AST) for Staphylococcus is crucial for determining the most effective antibiotics for treating infections caused by this bacterium. Here's an overview of the process: Methods Kirby-Bauer Disk Diffusion Test: This is a common method where antibiotic-impregnated disks are placed on an agar plate inoculated with the bacterial isolate. The plate is incubated, and the zone of inhibition (clear area around the disk) is measured to determine susceptibility. Minimum Inhibitory Concentration (MIC): This method determines the lowest concentration of an antibiotic that inhibits bacterial growth. Automated systems like VITEK 2 are often used for this purpose. E-test: A gradient method that provides an MIC value by placing a strip with a gradient of antibiotic concentration on an inoculated agar plate. Interpretation Sensitive: The bacterium is inhibited by the antibiotic at concentrations achievable in the body. Intermediate: The bacterium is inhibited at higher concentrations than usually achievable in the body. Resistant: The bacterium is not inhibited by the antibiotic at concentrations achievable in the body. Common Antibiotics Tested Methicillin: Used to identify Methicillin-Resistant Staphylococcus aureus (MRSA). Vancomycin: Often effective against MRSA. Linezolid: Another option for MRSA. Gentamicin: Commonly tested aminoglycoside. Ciprofloxacin: A fluoroquinolone. Clindamycin: Used for its coverage of anaerobes and certain Gram-positive bacteria. Clinical Relevance AST helps guide clinicians in selecting the most appropriate antibiotic therapy, reducing the risk of treatment failure and the development of antibiotic resistance #antimicrobialresistance #Antimicrobial