Ubiquitylation Of EYFP-tagged CENP-A (EYFP-CENP-A) l Protocol Preview скачать в хорошем качестве

Ubiquitylation Of EYFP-tagged CENP-A (EYFP-CENP-A) l Protocol Preview

Watch the Full Video at https://www.jove.com/v/61138/mass-spe.... Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A) - a 2 minute Preview of the Experimental Protocol Yohei Niikura, Lei Fang, Risa Kitagawa, Peizhao Li, Yao Xi, Ju You, Yan Guo, Katsumi Kitagawa Nanjing University, MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center; Medical School of Nanjing University, Jiangsu Key Laboratory of Molecular Medicine; UT Health Science Center San Antonio, Greehey Children's Cancer Institute, Department of Molecular Medicine; CENP-A ubiquitylation is an important requirement for CENP-A deposition at the centromere, inherited through dimerization between cell division, and indispensable to cell viability. Here we describe mass spectrometry analysis to identify ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A) protein. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments