Specificity Analysis-Protein Lysine Methyltransferases Using SPOT Peptide Arrays l Protocol Preview скачать в хорошем качестве

Specificity Analysis-Protein Lysine Methyltransferases Using SPOT Peptide Arrays l Protocol Preview

Watch the Full Video at https://www.jove.com/v/52203/specific.... Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays - a 2 minute Preview of the Experimental Protocol Srikanth Kudithipudi, Denis Kusevic, Sara Weirich, Albert Jeltsch Stuttgart University, Institute of Biochemistry; Peptide arrays synthesized by the SPOT method can be used to analyze the substrate specificity of Protein lysine methyltransferases (PKMTs) and to define the substrate spectrum of PKMTs to understand their biological role. This protocol describes how to synthesize peptide arrays, methylate them with PKMTs, and analyze the results. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments