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Watch the Full Video at https://www.jove.com/v/50404/a-compar.... A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions - a 2 minute Preview of the Experimental Protocol Mandy Muller, Patricia Cassonnet, Michel Favre, Yves Jacob, Caroline Demeret Institut Pasteur , Unité de Génétique, Papillomavirus et Cancer Humain (GPCH); Université Sorbonne Paris Cité, Cellule Pasteur; Dana Farber Cancer Institute, Center for Cancer Systems Biology (CCSB), Harvard Medical School, Department of Cancer Biology; This article focuses on the identification of high-confident interaction datasets between host and pathogen proteins using a combination of two orthogonal methods: yeast two-hybrid followed by a high-throughput interaction assay in mammalian cells called HT-GPCA. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments