Practical tips for mixing samples in eppendorf (microcentrifuge) tubes скачать в хорошем качестве

Practical tips for mixing samples in eppendorf (microcentrifuge) tubes

A few practical tips for mixing samples in eppendorf (microcentrifuge) tubes: via pipetting pipet up & down ~5 times (more if sample is viscous) just go to to and from the first stop (not all the way down (second stop) until the last time) use the right size tool for the job! set the volume to more than 50% the total volume in the tube, but less than the total volume too little and you won’t mix it too much and you’ll bring bubbles keep the tip of the tip just below the surface of the liquid this avoids liquid loss due to sticking to the outside of the tip this also prevents you overflowing make sure all the liquid goes out all the way alternatives; vortex (not if you have enzymes in there though!) finger vortex (flick tube to mix) - safe to do with enzymes too inverting tubes (only if larger volume) - can do multiple tubes in rack (held firmly down) NOT centrifuging - that doesn’t mix things! no matter the method, give a quick pulse spin after to draw liquid down off the sides of the tube and the inside of the cap before you lift the lid and have stuff splash out!   more practical lab tips & tricks: https://bit.ly/lab_tricks_page &    • Practical lab tips & tricks              more about all sorts of things: #365DaysOfScience All (with topics listed) 👉 http://bit.ly/2OllAB0 or search blog: http://thebumblingbiochemist.com