A Quick Tutorial on Integrating ChIP-Seq and Gene Expression Microarray Data Sets скачать в хорошем качестве

A Quick Tutorial on Integrating ChIP-Seq and Gene Expression Microarray Data Sets

GSE88896 is a study of the transcription factor TFEB and consists of two subseries. One is TFEB ChIP-Seq, and the other is microarray data profiling the effect of TFEB knockdown. This quick tutorial shows how to analyze the multi-omics datasets integratively. In this tutorial, we identify differentially expressed genes by TFEB knockdown and separate them into two groups: those with TFEB peaks near their TSS and those without. They are supposed to be the direct target of TFEB and the indirect effects. We also compare experimentally detected target genes with predicted targets from the DNA sequence. Although you can follow the steps to grab the workflow, we have omitted some of the detailed steps in this movie. We instead provide the SOA file so that you can import the data and easily follow the operations. It also enables further analysis by yourselves. Please forgive that the SOA file doesn’t include MSigDB-related data due to the license issue. You can import the SOA file from “Import SSA/SOA File...” under the “File” menu. After restarting the Subio Platform, load the series from the “Series” tab in the lower panel of the “Data Manager.” So, enjoy your exploring. https://www.subioplatform.com/info_ca... Reference: https://www.ncbi.nlm.nih.gov/geo/quer...