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Watch the Full Video at https://www.jove.com/v/51906/collecti.... Collection, Isolation, and Flow Cytometric Analysis of Human Endocervical Samples - a 2 minute Preview of the Experimental Protocol Jennifer A. Juno, Genevieve Boily-Larouche, Julie Lajoie, Keith R. Fowke University of Manitoba, Department of Medical Microbiology; University of Manitoba, Department of Community Health Sciences; The use of cytobrush sampling to collect lymphocytes and monocytes from the endocervix is a minimally invasive technique that provides samples for analysis of female genital tract immunity. In this protocol, we describe the collection of cytobrush samples and immune cell isolation for flow cytometry assays. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments