Polysome Fractionation & Analysis: Mammalian Translatomes On Genome-Wide Scale l Protocol Preview скачать в хорошем качестве

Polysome Fractionation & Analysis: Mammalian Translatomes On Genome-Wide Scale l Protocol Preview

Watch the Full Video at https://www.jove.com/v/51455/polysome.... Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale - a 2 minute Preview of the Experimental Protocol Valentina Gandin, Kristina Sikström, Tommy Alain, Masahiro Morita, Shannon McLaughlan, Ola Larsson, Ivan Topisirovic McGill University, Lady Davis Institute and Department of Oncology; Karolinska Institutet, Department of Oncology-Pathology; McGill University, Goodman Cancer Centre and Department of Biochemistry; Ribosomes play a central role in protein synthesis. Polyribosome (polysome) fractionation by sucrose density gradient centrifugation allows direct determination of translation efficiencies of individual mRNAs on a genome-wide scale. In addition, this method can be used for biochemical analysis of ribosome- and polysome-associated factors such as chaperones and signaling molecules. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments