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Reference: https://app.jove.com/t/2839/dissectio... Dissection of the adult zebrafish kidney is a critical procedure in understanding the organ's structure and function, as well as its role in various physiological processes. The zebrafish, a model organism widely used in biological research, offers unique advantages due to its transparent embryos and rapid development. The adult kidney, known as the pronephros, is composed of several distinct regions, including the glomerulus, tubules, and collecting ducts. Each of these components plays a vital role in filtration, reabsorption, and excretion, making the dissection process essential for studying renal physiology and pathology. To begin the dissection, it is important to properly anesthetize the zebrafish to minimize stress and ensure humane treatment. Once the fish is adequately anesthetized, it is placed on a dissection tray, and the abdominal cavity is carefully opened using fine scissors or a scalpel. The kidneys, located along the dorsal side of the body cavity, can be identified by their characteristic shape and color. Careful manipulation is required to avoid damaging surrounding tissues, as the kidneys are often closely associated with other organs such as the liver and intestines. Once exposed, the kidneys can be removed for further examination or analysis, allowing researchers to investigate their cellular architecture and function. Following dissection, various techniques can be employed to analyze the kidney tissue. Histological staining methods, such as hematoxylin and eosin, can be used to visualize the cellular components under a microscope, revealing important information about the organization and health of the kidney. Additionally, molecular techniques, including in situ hybridization and immunohistochemistry, can provide insights into gene expression patterns and protein localization within the kidney. This comprehensive approach to studying the adult zebrafish kidney not only enhances our understanding of renal biology but also contributes to broader research efforts in developmental biology, genetics, and disease modeling.