Two-Step Affinity Purification Yielding Full-Length Purified Proteins l Protocol Preview скачать в хорошем качестве

Two-Step Affinity Purification Yielding Full-Length Purified Proteins l Protocol Preview

Watch the Full Video at https://www.jove.com/v/50320/gst-his-.... GST-His purification: A Two-step Affinity Purification Protocol Yielding Full-length Purified Proteins - a 2 minute Preview of the Experimental Protocol Ranjan Maity, Joris Pauty, Jana Krietsch, Rémi Buisson, Marie-Michelle Genois, Jean-Yves Masson Hôtel-Dieu de Québec, Genome Stability Laboratory, Oncology Axis; In the present protocol, we demonstrate a highly efficient and cost-effective small-scale protein purification method, which allows purification of recombinant proteins by uniquely combining a cleavable GST-tag and a small His-tag. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments