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Welcome to Science With Tal! In this clip of the The Neuromuscular Junction as the model of the chemical synapse video, we will cover the experimental basis for calcium dependence. Moreover, calcium is often said to be necessary and sufficient for release and this video will explain this terminology. To improve the quality of my content, I highly value the feedback from the viewer so do not hesitate to give any feedback in the comment section. Timestamps 0:00 Introduction 0:16 Motivation for calcium 2:45 Experimental basis for calcium dependence 5:35 Calcium as necessary and sufficient explained 6:09 Conclusion Resources used Here is a list of the resources that I’ve used to produce this video. (Author: title resource) Dale Purves: Neuroscience (6th edition) Eric Kandel: Principles of neural science (6th edition) Robertson, J. David: Some Features of the Ultrastructure of Reptilian Skeletal Muscle Heuser, J E et al. : Synaptic vesicle exocytosis captured by quick freezing and correlated with quantal transmitter release Heuser, J E and T S Reese: Evidence for recycling of synaptic vesicle membrane during transmitter release at the frog neuromuscular junction Llinás, R and Y Yarom: Properties and distribution of ionic conductances generating electroresponsiveness of mammalian inferior olivary neurones in vitro Katz, B, and R Miledi: A study of synaptic transmission in the absence of nerve impulses BOYD, I A,and A R MARTIN: The end-plate potential in mammalian muscle DEL CASTILLO, J, and B KATZ: Quantal components of the end-plate potential To have more information on these resources, you can refer to the conclusion section where a more formal citation is provided. Video credits Writing: Tal Klimenko Voice: Tal Klimenko Animations: Tal Klimenko Drawings: Tal Klimenko Editing: Tal Klimenko Introductory jingle: Thierry Du Sablond Conclusion music: lukrembo - jay ( • lukrembo - jay (no copyright music) )