Rapid urease test| Upper gastrointestinal endoscopy| Helicobacter pylori| Dr. Pandiaraja скачать в хорошем качестве

Rapid urease test| Upper gastrointestinal endoscopy| Helicobacter pylori| Dr. Pandiaraja

Rapid urease test, also known as the CLO test (Campylobacter-like organism test), is a rapid diagnostic test for diagnosis of Helicobacter pylori. The basis of the test is the ability of H. pylori to secrete the urease enzyme, which catalyzes the conversion of urea to ammonia and carbon dioxide The test is performed at the time of gastroscopy. A biopsy of mucosa is taken from the antrum of the stomach, and is placed into a medium containing urea and an indicator such as phenol red. The urease produced by H. pylori hydrolyzes urea to ammonia, which raises the pH of the medium, and changes the color of the specimen from yellow (NEGATIVE) to red (POSITIVE). Among different kinds of rapid urease tests (liquid-based, gel-based, dry cool) there is a design type with single-layer sensitive element — a layer impregnated simultaneously with urea and an indicator composition. Such a design bears the risk of false-positive result due to the pH value of the gastric biopsy when it is placed on the sensitive element. Excessive salivation and alkaline bile reflux into the stomach can shift the pH value of the biopsy of the stomach towards alkaline. Drugs that reduce the acidity of the stomach, also contribute to false positive results resulting from the alkalization. In each of these cases, the pH of the biopsy will be shifted to the alkaline side. All of these factors may trigger a non-targeted coloration of the sensitive element in a single-layer rapid urease test. The indicator would change color not in the course of the enzymatic reaction, which then causes alkalization of the medium as a result of ammonia formation, but under the effect of the pH of the gastric biopsy.