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Step-by-Step Guide to Horse Castration Procedure and Post-Operative Care. This video provides a step-by-step guide on how to castrate a horse stallion. It begins by stressing the importance of having a veterinarian assess the horse before the procedure and discussing the options for castration surgery. Depending on the horse's health, the surgery may be done under sedation or full anesthesia in a clinical setting. The veterinarian will also decide whether to perform the surgery with the horse standing up or lying down. The procedure involves sedating the horse, cleaning the scrotal area, injecting local anesthesia into each testicle, making an incision, popping out the testicles, applying emasculators, sealing the blood vessels, and checking for signs of hemorrhage. The procedure is repeated on the other side for the other testicle, and the incisions are left open to heal by secondary intention. After the surgery, the horse needs to rest in its stall for about 24 hours before getting light exercise, and the scrotal area must be kept clean.