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Watch the Full Video at https://www.jove.com/v/59749/adult-mo.... Adult Mouse Digit Amputation and Regeneration: A Simple Model to Investigate Mammalian Blastema Formation and Intramembranous Ossification - a 2 minute Preview of the Experimental Protocol Lindsay A. Dawson, Regina Brunauer, Katherine N. Zimmel, Osama Qureshi, Alyssa R. Falck, Patrick Kim, Connor P. Dolan, Ling Yu, Yu-Lieh Lin, Benjamin Daniel, Mingquan Yan, Ken Muneoka Texas A&M University, Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences; Texas A&M University, Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences; University of Mississippi Medical Center, Department of Neurosurgery; Here, we present a protocol of adult mouse terminal phalanx amputation to investigate mammalian blastema formation and intramembranous ossification, analyzed by fluorescent immunohistochemistry and sequential in-vivo microcomputed tomography. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments