Laser Microdissection To Uncover Regional Transcriptomics: Human Kidney Tissue l Protocol Preview скачать в хорошем качестве

Laser Microdissection To Uncover Regional Transcriptomics: Human Kidney Tissue l Protocol Preview

Watch the Full Video at https://www.jove.com/v/61371/applicat.... Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue - a 2 minute Preview of the Experimental Protocol Daria Barwinska, Michael J. Ferkowicz, Ying-Hua Cheng, Seth Winfree, Kenneth W. Dunn, Katherine J. Kelly, Timothy A. Sutton, Brad H. Rovin, Samir V. Parikh, Carrie L. Phillips, Pierre C. Dagher, Tarek M. El-Achkar, Michael T. Eadon, Indiana University School of Medicine, Department of Medicine; Indiana University School of Medicine, Department of Cellular & Integrative Physiology; Ohio State University Wexner Medical Center, Division of Nephrology, Department of Medicine; Indiana University School of Medicine, Division of Pathology; We describe a protocol for laser microdissection of sub-segments of the human kidney, including the glomerulus, proximal tubule, thick ascending limb, collecting duct and interstitium. The RNA is then isolated from the obtained compartments and RNA sequencing is carried out to determine changes in the transcriptomic signature within each sub-segment. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments