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Watch the Full Video at https://www.jove.com/v/56227/assessme.... Assessment of the Anticoagulant and Anti-inflammatory Properties of Endothelial Cells Using 3D Cell Culture and Non-anticoagulated Whole Blood - a 2 minute Preview of the Experimental Protocol Riccardo Sfriso, Anjan Bongoni, Yara Banz, Nikolai Klymiuk, Eckhard Wolf, Robert Rieben University of Bern, Department of Clinical Research; University of Bern, Graduate School for Cellular and Biomedical Sciences; St. Vincent's Hospital Melbourne, Immunology Research Centre; University of Bern, Institute of Pathology; Ludwig-Maximilian University, Institute of Molecular Animal Breeding and Biotechnology; We present an in vitro model which allows the study and analysis of coagulation in whole, non-anticoagulated blood. Anticoagulation in the system depends on the natural anticoagulation effect of healthy endothelial cells and endothelial cell activation will result in clotting. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments