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Expression of the Recombinant DNA. A clone can be a large number of molecules (i.e. cloned genes) or cells (i.e. cloned bacteria) or organisms that are identical to an original specimen. Plasmid Entry. Bacterial cells take up recombinant plasmid, especially, if they are treated with calcium chloride to make them more permeable. Cloning of plasmids. Thereafter, as the cell reproduces, a bacterial clone forms and each new cell contains at least one plasmid. Each bacteria. Therefore, each of the bacteria contains the gene of interest, Which will express itself and make a product. From this bacterial clone, the cloned gene can be isolated for further analysis, or protein product can be separated. Use of Viruses. Besides plasmids, the DNA of bacterial viruses (for example, lambda phage) can also be used as a vector. After lambda phage attaches to a host bacterium, recombinant DNA is released from the virus and enters the bacterium. Here, it will direct the reproduction of many more viruses. Each virus in bacteriophage clone contains a copy of the gene being cloned.