Fibroblast-Derived 3D Matrix System in Endothelial Tube Assay | Protocol Preview скачать в хорошем качестве

Fibroblast-Derived 3D Matrix System in Endothelial Tube Assay | Protocol Preview

Watch the Full Video at https://www.jove.com/v/60304/fibrobla.... Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay - a 2 minute Preview of the Experimental Protocol Cristina Galindo-Pumariño, Alberto Herrera, Alberto Muñoz, Alfredo Carrato, Mercedes Herrera, Cristina Peña Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), CIBERONC, Medical Oncology Department; Hospital Universitario Puerta de Hierro de Majadahonda, Department of Medical Oncology; Consejo Superior de Investigaciones Científicas - Universidad Autónoma de Madrid, CIBERONC, Instituto de Investigaciones Biomédicas Alberto Sols; Karolinska Institutet, Department of Oncology & Pathology; The aim of this method is to obtain fibroblast-derived 3D matrices as a natural scaffold for subsequent cellular assays. Fibroblasts are seeded in a pre-treated culture plate and stimulated with ascorbic acid for matrix generation. Matrices are decellularized and blocked to culture relevant cells (e.g., endothelial cells). Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments