Understanding CY5 Phosphoramidite in 60 Seconds скачать в хорошем качестве

Understanding CY5 Phosphoramidite in 60 Seconds

At SBS Genetech, we offer high-quality Phosphoramidite. Explore more at https://www.sbsgenetech.com/portfolio.... Cy5 phosphoramidite is a widely used fluorescent dye derivative for labeling oligonucleotides during DNA synthesis. This cyanine dye, with a molecular weight of 816.49 and formula C47H67N5ClO3P, exhibits spectral properties ideal for far-red emission applications. It has an excitation maximum at 646 nm and an emission maximum at 662 nm, with a high extinction coefficient of 250,000 M^-1 cm^-1. Cy5 phosphoramidite is commonly employed in qPCR, DNA sequencing, and other fluorescence-based techniques requiring far-red emission or multiplex detection. During solid-phase oligonucleotide synthesis, it can be incorporated typically at the 5' end, with a recommended 6-minute coupling time. The phosphoramidite group's attachment to a secondary carbon provides enhanced stability against Arbuzov rearrangement. Mild deprotection conditions are advised, such as 48 hours at 4°C or using ultramild protective groups. For optimal preservation, Cy5 phosphoramidite should be stored at -20°C in the dark, although it can withstand room temperature for brief periods during transportation. Its solubility in acetonitrile, a common solvent in oligonucleotide synthesis, further contributes to its practicality in research applications.