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In this video, we will explore the chromatographic separation of coumarins and saponins. Chromatographic separation is a technique used to separate and analyze different compounds in a mixture. Coumarins are a class of organic compounds known for their aromatic and anticoagulant properties. Saponins, on the other hand, are natural compounds found in plants that have diverse biological activities. By using chromatography, we can separate and identify specific coumarins and saponins present in a sample. This separation is achieved by exploiting the differences in their physical and chemical properties. The chromatographic process involves a stationary phase and a mobile phase. The stationary phase is a solid or liquid material where the separation takes place. The mobile phase is a liquid or gas that carries the sample through the stationary phase. As the sample moves through the stationary phase, different compounds interact differently, leading to their separation.