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Expanded Bed Adsorption (EBA) chromatography is a powerful technique used in bioprocessing, particularly for capturing target biomolecules directly from crude feedstock like cell lysates or fermentation broths. Unlike traditional chromatography, which requires prior clarification steps, EBA enables direct processing of unclarified samples by combining solid-liquid separation and adsorption in a single step. This results in a more streamlined and efficient purification process. In the keynote presented by Prof. Marcelo Fernandes-Lahore at ESBES 2016 in Dublin, the focus was on the advanced principles and real-world applications of EBA. Prof. Lahore highlighted how the technique leverages fluidized beds and specially designed adsorbent particles to allow for the efficient capture of biomolecules while handling impurities such as cell debris and particulates. This significantly reduces processing time and improves yield, making EBA a valuable tool in the production of biopharmaceuticals. The presentation also covered optimization strategies for EBA systems, showcasing their versatility in industrial applications for purifying proteins, antibodies, and other therapeutic molecules. Prof. Lahore’s insights into this method demonstrated its potential to enhance downstream processing by simplifying workflows and reducing costs, making it a key technology in modern biomanufacturing. Expanded Bed Adsorption, EBA chromatography, bioprocessing, downstream processing, biopharmaceuticals, protein purification, chromatography, Marcelo Fernandes Lahore, ESBES 2016, biomanufacturing, fluidized bed, protein capture, biopharma purification, industrial biotechnology, purification techniques, expanded bed adsorption, expanded bed adsorption chromatography, expanded bed chromatography