MHC-tetramer Staining and Quantitative Analysis of CD8 T Cells | Protocol Preview скачать в хорошем качестве

MHC-tetramer Staining and Quantitative Analysis of CD8 T Cells | Protocol Preview

Watch the Full Video at https://www.jove.com/v/56130/in-situ-.... In Situ MHC-tetramer Staining and Quantitative Analysis to Determine the Location, Abundance, and Phenotype of Antigen-specific CD8 T Cells in Tissues - a 2 minute Preview of the Experimental Protocol Shengbin Li, Gwantwa Mwakalundwa, Pamela J. Skinner University of Minnesota, Department of Veterinary and Biomedical Sciences; Here, we describe a method that combines in situ MHC-tetramer staining with immunohistochemistry to determine localization, phenotype, and quantity of antigen-specific T cells in tissues. This protocol is used to determine the spatial and phenotypic characteristics of antigen-specific CD8 T cells relative to other cell type and structures in tissues. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments