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In this video, we explore the fascinating world of fluorescence and total internal reflection by synthesizing fluorescein and demonstrating its unique properties. Fluorescein is a fluorescent compound that emits bright green light when exposed to UV light. We take you through the synthesis process step-by-step and show you how to purify and characterize the compound. Once we have our purified fluorescein, we use it to demonstrate total internal reflection, a phenomenon that occurs when light is reflected back into a medium rather than passing through it. By setting up a simple optical setup with a prism and a laser, we show how fluorescein can be used to visualize the critical angle of total internal reflection and create stunning optical effects. Whether you're a chemistry enthusiast, a science teacher, or just someone who loves learning new things, this video is sure to fascinate and inspire you. Join us on this journey of discovery and see the beauty of fluorescence and total internal reflection come to life! support me through patreon / akhilthechemist or Paypal →https://www.paypal.me/akhilthechemist