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Recombinant DNA technologies have been used extensively in basic biological research and in the applied sciences, such as medical applications. Although plasmids have been widely used up until now, engineering of whole genomes is favoured. Unfortunately, engineering genomes is complicated. Since we don’t fully understand genomes and their functions, engineering them is often not straightforward and unwanted effects may occur. In this context, the field of synthetic biology emerged with the aim of optimizing these systems by incorporating engineering principles. But this field is currently facing two major challenges: accessibility and reproducibility. In this video, Carolyn Bayer from the Technical University of Denmark, talks about these issues and about the Standardized Genome Engineering Architecture or SEGA, that aims to tackle these challenges. This video is a part of the European Project "BioRoboost: Fostering Synthetic Biology standardisation through international collaboration". This project has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement N820699