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Watch the Full Video at https://www.jove.com/v/3587/a-3d-syst.... A 3D System for Culturing Human Articular Chondrocytes in Synovial Fluid - a 2 minute Preview of the Experimental Protocol Joshua A. Brand, Timothy E. McAlindon, Li Zeng Tufts University School of Medicine, Department of Anatomy and Cellular Biology; Tufts Medical Center, Department of Rheumatology; A 3D system of culturing human articular chondrocytes in high levels of synovial fluid is described. Synovial fluid reflects the most natural microenvironment for articular cartilage, and can be easily obtained and stored. This system thus can be used for studying cartilage regeneration and for screening therapeutics for treating arthritis. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments