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Watch the Full Video at https://www.jove.com/v/51425/reconsti.... Reconstitution Of β-catenin Degradation In Xenopus Egg Extract - a 2 minute Preview of the Experimental Protocol Tony W. Chen, Matthew R. Broadus, Stacey S. Huppert, Ethan Lee Vanderbilt University Medical Center, Department of Cell and Developmental Biology and Program in Developmental Biology; Cincinnati Children's Hospital Medical Center, Division of Gastroenterology, Hepatology & Nutrition and Division of Developmental Biology; Vanderbilt University School of Medicine, Vanderbilt Ingram Cancer Center; A method is described for analyzing protein degradation using radiolabeled and luciferase-fusion proteins in Xenopus egg extract and its adaptation for high-throughput screening for small molecule modulators of protein degradation. Visit https://www.jove.com?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments