Webinar: FACS OFF! Cellenion & Biosero скачать в хорошем качестве

Webinar: FACS OFF! Cellenion & Biosero

FACS OFF! Autonomous & High Throughput Single Cell Cloning B-cell cloning is an industry standard method for reliable and reproducible production of monoclonal antibodies (mAb). A typical journey in mAb development involves B cells isolation prior to screening. For years, FACS has been the method of choice for semi-automated seeding of single cells into microwell plates. However, this method suffers from low throughput due to inherent difficulties to automate start up, calibration, sample/target exchange processes. Furthermore, FACS is labour-intensive as it requires continuous presence of skilled operator to monitor and troubleshoot the process. Hence, the need of an alternative approach that addresses the aforementioned shortcomings that are: throughput, clonability, and high operating costs. In this webinar, we will present how cellenONE HT, an image-based single cell isolation platform, is fully automated using Green Button Go Scheduler so that it operates continuously without the need for an operator, effectively enabling 24/7 unattended single cell plating from one or multiple samples. Speakers 🗣️ 🎤 Mathieu Bennet, CTO, Cellenion Mathieu leads the product development at Cellenion where the emerging need of researchers and scientists to automate single cell workflows is translated into solutions such as the cellenONE HT. Before joining Cellenion 6 years ago, he graduated with a PhD on microfluidic devices from the University of Edinburgh (UK) and spent 7 years on the biophysical study of biomaterials at the MPIKG in Potsdam (Germany). 🎤 Dustin Whitson, Applications Consulting Scientist, Biosero Dustin Whitson is an Applications Consulting Scientist at Biosero where he works with scientists from labs around the world to design solutions for automating science. He has spent the last 6 years specializing in delivering automation into the lab setting with applications experience at Biosero and Hamilton Robotics. Frequently Asked Questions (FAQ) Question 1: Can the system tell the difference between live and dead cells? Yes, the cellenONE HT is equipped with fluorescence, so you can have a usual red/green marker, which allows you to sort. So if you want to sort only live cells, you can do that. Question 2: Does cellenONE HT work with samples in 96 well plates? Yes, samples can be placed in 96 well plates. Question 3 : Why is the clonal outgrowth better with the cellenONE HT? This is thanks to our proprietary dispensing technology based on a very gentle piezo-acoustic stimuli to generate the drops. Question 4: What is the minimum sample volume for a campaign? The dead volume of the sample container is less than 2 µL and we recommend a cell density of around 150 cells/µL. For isolating cells in 4 x 384 well plates we recommend a volume of around 15 µL. Question 5: Can cellenONE HT be operated without Green Button Go (Scheduler)? Yes, the cellenONE HT comes with its own proprietary software that can be used to operate our technology as a standalone device. Question 6: What is the volume and variation of the drop? The drop volume is around 350 pL and the CV below 2%