Primary Culture & Plasmid Electroporation Of Murine Organ Of Corti. l Protocol Preview скачать в хорошем качестве

Primary Culture & Plasmid Electroporation Of Murine Organ Of Corti. l Protocol Preview

Watch the Full Video at https://www.jove.com/v/1685/primary-c.... Primary Culture and Plasmid Electroporation of the Murine Organ of Corti. - a 2 minute Preview of the Experimental Protocol Mark Parker, Aurore Brugeaud, Albert S. B. Edge Harvard Medical School, Department of Otology and Laryngology; Massachusetts Eye and Ear Infirmary, Eaton-Peabody Laboratory; Emerson College, Department of Communication Sciences and Disorders; Harvard, Program in Speech and Hearing Bioscience and Technology, Division of Health Science and Technology; This procedure describes a method for the isolation and culture of the murine organ of Corti with or without the spiral limbus and spiral ganglion neurons. We also demonstrate a method for the expression of an exogenous reporter gene in the organ of Corti explant by electroporation. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments