Ex-ovo Electroporation for Gene Transfer for Cell Culture Studies | Protocol Preview скачать в хорошем качестве

Ex-ovo Electroporation for Gene Transfer for Cell Culture Studies | Protocol Preview

Watch the Full Video at https://www.jove.com/v/52002/efficien.... Efficient Gene Transfer in Chick Retinas for Primary Cell Culture Studies: An Ex-ovo Electroporation Approach - a 2 minute Preview of the Experimental Protocol M. Natalia Vergara, Christian Gutierrez, M. Valeria Canto-Soler The Johns Hopkins University School of Medicine, Wilmer Eye Institute; Embryonic chick retinal cell cultures constitute valuable tools for the study of photoreceptor biology. We have developed an efficient gene transfer technique based on ex ovo plasmid electroporation of the retinas prior to culture. This technique considerably increases transfection efficiencies over currently available protocols, making genetic manipulation feasible for this system. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments