🪀🛑 Protein Microarray: MICROARRAY DATA ANALYSIS скачать в хорошем качестве

🪀🛑 Protein Microarray: MICROARRAY DATA ANALYSIS

Microarrays are solid supports (usually glass slides) upon which 10s, 100s or thousands of DNA, proteins or antibodies are printed in tiny spots in defined positions. Each spot contains a known DNA sequence or gene or protein or antibody. To analyse microarrays, do the follow: 1. Optimise for a scan on your microarray scanner that has no saturated pixels in the spots on the microarray slide. 2. Initiate the microarray data analysis process using the Genepix Array List (GAL) file. 3. Align the microarray blocks and adjust the spots (locations within each block) as needed. Use the microarray software to extract quantified fluorescent intensities for each spot in a block. Microarray Data normalisation: The positive and negative controls on each block are used to decide what a high and low positive looks like, and what a no-signal fluorescence (negative) looks like. The microarray positive controls on each block within a slide act as INTRA slide normalisation. Your included QC control or microarray standard 3 (recommended for InterSlide Variation Correction Tool) can be used to normalise from slide to slide. . . . . . // ADWOA I'm Adwoa (Adwoa Biotech), a Biotechnology graduate. I’ve worked in medical research for years and want to be useful to people new to the lab life. This channel takes you through some of the techniques and concepts I've learnt working as a Research Assistant. Hopefully it helps if you're new to the topic/technique. This video is about microarray, microarray analysis, microarray technology, microarray explained, microarray data analysis, microarray data, microarray procedure, microarray hybridisation, DNA microarray and protein microarray.