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How to Make Plates, Slants, and Liquid Culture for Mushroom Cultivation | Agar Recipe | Lab Skills

Mycology Lab Supplies: https://bit.ly/3mZTUUs In this video, learn how to make plates, slants, and liquid culture for mushroom cultivation & preservation with North Spore’s head mycologist, Jon Carver! He’ll show you how to prepare three different types of media typically found in mycological settings. This includes MYA (malt, yeast, agar), PDA (potato, dextrose, agar) and media for liquid culture. Included in the video are recipes for agar preparation and liquid culture media. These are simple, universal recipes that can be expanded on depending on the application. Although we shot this video in the North Spore lab, many of these techniques can be adapted to home or low-tech environments. For more information on these processes including a complete list of lab materials, ingredients and low-tech alternatives, click the link below! Making Plates, Slants, and Liquid Culture Blog: https://northspore.com/blogs/the-blac... Liquid Culture Multiplier Kit: https://northspore.com/products/liqui... Mycology Lab Starter Set Kit + NocBox Still Air Box: https://northspore.com/products/mycol... Pre-made Agar Plates: https://northspore.com/collections/la... Pre-made Agar Slants: https://northspore.com/collections/la... North Spore Culture Bank: https://northspore.com/collections/cu... All American 75X Electric Steam Autoclave Sterilizer: https://northspore.com/collections/st... Thanks for watching! Let us know in the comments what you want to see next! Index 00:00 - Introduction 00:27 - Liquid vs solid media 02:50 - PDA, MYA, and liquid media 03:34 - How to make liquid culture media 04:08 - How to make MYA for plates 04:46 - How to make PDA for slants 05:45 - How to prepare slants for the pressure cooker 06:50 - Pressure cooking media 08:26 - Working in the lab 09:11 - Creating the "slant" in slants and pouring plates 13:31 - Using culture media 13:51 - How to inoculate an agar plate using a slant 14:29 - How to inoculate a slant using an agar plate 15:03 - How to inoculate liquid culture using solid media 15:20 - How to inoculate liquid culture using a culture syringe Music “Even Though it Hurts” (instrumental version) - spring gang “Principles” - _91nova “I Should Let Go” - Rambutan “By The Palms” - Gregory David “Dreamaway” - dream “Pickup Sticks” (instrumental version) - Pandaraps “Bringing Me Down” - Ooyy