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Watch the Full Video at https://www.jove.com/v/60155/medium-p.... Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions - a 2 minute Preview of the Experimental Protocol Andreas O. Wagner, Rudolf Markt, Mira Mutschlechner, Nina Lackner, Eva M. Prem, Nadine Praeg, Paul Illmer Universität Innsbruck, Department of Microbiology; As obligate anaerobic organisms are unable to grow upon oxygen exposure, the use of anaerobic culturing techniques is indispensable. Here, we demonstrate a simple and effective method to cultivate a mixed culture derived from a biogas plant from media preparation to gas and volatile fatty acid quantification. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments