MHC I Tetramer Staining used for Quantification of CD8+ T Cells | Protocol Preview скачать в хорошем качестве

MHC I Tetramer Staining used for Quantification of CD8+ T Cells | Protocol Preview

Watch the Full Video at https://www.jove.com/v/58680/simultan.... Simultaneous Quantification of Anti-vector and Anti-transgene-Specific CD8+ T Cells Via MHC I Tetramer Staining After Vaccination with a Viral Vector - a 2 minute Preview of the Experimental Protocol Sarah Wilmschen, Zoltan Banki, Dorothee von Laer, Janine Kimpel Medical University of Innsbruck, Division of Virology, Department of Hygiene, Microbiology, Social Medicine; Here, we present a protocol for the ex vivo qualitative detection of antigen-specific CD8+ T cells. Analysis is possible with single cell suspensions from organs or from small amounts of blood. A broad range of studies require the analysis of cytotoxic T cell responses (vaccination and cancer immunotherapy studies). Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments