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Watch the Full Video at https://www.jove.com/v/3886/micropipe.... Micropipette Aspiration of Substrate-attached Cells to Estimate Cell Stiffness - a 2 minute Preview of the Experimental Protocol Myung-Jin Oh, Frank Kuhr, Fitzroy Byfield, Irena Levitan University of Illinois, Section of Respiratory, Critical Care and Sleep Medicine, Department of Medicine; University of Pennsylvania , Institute for Medicine and Engineering; Here we describe a quick and simple method to measure cell stiffness. The general principle of this approach is to measure membrane deformation in response to well-defined negative pressure applied through a micropipette to the cell surface. This method provides a powerful tool to study biomechanical properties of substrate-attached cells. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments