Microbiome Informatics: OTUs vs. ASVs скачать в хорошем качестве

Microbiome Informatics: OTUs vs. ASVs

In this video, we review the basic concepts of OTU and ASV-based methods for targeted microbiome sequencing resolution. Topics will include an introduction to targeted microbiome sequencing, how both OTU and ASV are generated, and the advantages and disadvantages of each method for 16S and other targeted sequencing studies. Read more about OTUs vs ASVs, including their pros and cons for difficult sample types here: Microbiome Informatics: https://www.zymoresearch.com/blogs/bl... 0:00 Introduction 0:14 Targeted Sequencing 0:29 A Potential Target for Sequencing 1:14 Finding an Ideal Target Sequence 1:51 16S Sequencing Challenges 2:34 The Addition of Noise 3:10 A Quick Note 3:42 Clustering (Blurring) 3:56 Clustering (De Novo) 4:29 Clustering (Open Reference) 4:51 Clustering (Closed Reference) 5:22 Operational Taxonomic Unit (OTU) Approach 6:11 And Differences... 6:14 And an Error Model for the Run... 7:32 Adding More Samples 7:59 What Else Can Change? 8:49 Amplicon Sequence Variant (ASV) Approach What is the statistical support for each sequence's existence? #Microbiome #OTUvsASV #16SSequencing #Microbiomics #Bioinformatics #ZymoResearch #DNASequencing #Metagenomics --------------------------------------------------------------------------------------------- Want to talk to a scientist? Contact us: https://www.zymoresearch.com/pages/co... Website: http://www.zymoresearch.com LinkedIn:   / zymo-research-corp   Instagram:   / zymo_research   Twitter:   / zymoresearch   Bluesky: https://bsky.app/profile/zymoresearch...