Electroporation: Chick Midbrain-Gene Function: Dopaminergic Neuron Development l Protocol Preview скачать в хорошем качестве

Electroporation: Chick Midbrain-Gene Function: Dopaminergic Neuron Development l Protocol Preview

Watch the Full Video at https://www.jove.com/v/4017/in-ovo-el.... In ovo Electroporation in Chick Midbrain for Studying Gene Function in Dopaminergic Neuron Development - a 2 minute Preview of the Experimental Protocol Ben Yang, Lauren B. Geary, Yong-Chao Ma Children's Hospital of Chicago Research Center, Northwestern University Feinberg School of Medicine; Northwestern University Feinberg School of Medicine, Departments of Pediatrics, Neurology and Physiology; To assess the function and the regulation of genes during the development of midbrain dopaminergic neurons, we describe a method that involves in ovo electroporation of plasmid DNA constructs into embryonic chick ventral midbrain dopaminergic neuron progenitors. This technique can be used to achieve efficient expression of genes of interest to study different aspects of midbrain development and dopaminergic neuron differentiation. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments