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Watch the Full Video at https://www.jove.com/v/2154/an-ex-ovo.... An ex-ovo Chicken Embryo Culture System Suitable for Imaging and Microsurgery Applications - a 2 minute Preview of the Experimental Protocol Huseyin C. Yalcin, Akshay Shekhar, Ajinkya A. Rane, Jonathan T. Butcher Cornell University, Department of Biomedical Engineering; Dogus University, Current Address: Mechanical Engineering Department; In this article, we present a simple methodology to enable long-term ex-ovo avian embryo culture. This technique is ideal for longitudinal experimentation requiring complete optical accessibility and/or sterile transportation in avian embryos. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments