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Watch the Full Video at https://www.jove.com/v/56712/dissecti.... Dissection and Explant Culture of Murine Allantois for the In Vitro Analysis of Allantoic Attachment - a 2 minute Preview of the Experimental Protocol Kerstin Hadamek, Angelika Keller, Antje Gohla University of Würzburg, Institute of Pharmacology and Toxicology; University of Würzburg, Rudolf Virchow Center for Experimental Biomedicine; We describe an in vitro assay to model chorioallantoic attachment, the first step in placenta formation. The protocol demonstrates the dissection and explant culture of murine allantoides on immobilized α4β1 integrin. Allantois attachment is evaluated microscopically at pre-determined time points. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments