Viral-mediated Labeling and Transplantation of MGE Cells | Protocol Preview скачать в хорошем качестве

Viral-mediated Labeling and Transplantation of MGE Cells | Protocol Preview

Watch the Full Video at https://www.jove.com/v/52740/viral-me.... Viral-mediated Labeling and Transplantation of Medial Ganglionic Eminence (MGE) Cells for In Vivo Studies - a 2 minute Preview of the Experimental Protocol Daniel Vogt, Pei-Rung Wu, Shawn F. Sorrells, Christine Arnold, Arturo Alvarez-Buylla, John L. R. Rubenstein University of California San Francisco, Department of Psychiatry; University of California San Francisco, Department of Neurological Surgery; GABAergic cortical interneuron progenitors disperse, develop and synaptically integrate into a host cortex after transplantation. These cells can be easily transduced before transplantation for in vivo studies of genetically modified GABAergic precursors. Here, we show viral labeling techniques to target specific interneuron subgroups using existing Cre lines and Cre-dependent reporters. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments